[Detection of polyphosphatase activity in isolated Saccharomyces cerevisiae nuclei]

Intact nuclei have been isolated from cells of a diploid strain of Saccharomyces cerevisiae. The isolated nuclei were free from cytoplasmic, mitochondrial and vacuolar marker enzymes. The protein to DNA ratio (w/w) was 11. Pyrophosphatase, tripolyphosphatase and exopolyphosphatase activities have been found in S. cerevisiae nuclei for the first time and were equal to 400, 130 and 55 mU/mg of protein, respectively.