Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Toxoplasmosis in nude mice

R E Lindberg, J K Frenkel

    The Journal of Parasitology
    |April 1, 1977
    PubMed
    Summary

    Cellular immunity, not antibodies, is key for mice to develop Toxoplasma gondii immunity. Thymus cell transplants enabled nude mice to gain immunity during drug treatment, highlighting the role of cellular immunity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Relative reversal by vitamins (rho-aminobenzoic, folic, and folinic acids) of the effects of sulfadiazine and pyrimethamine on Toxoplasma, mouse and man.

    Antibiotics & chemotherapy (Northfield, Ill.)·2014
    Same author

    Reflection on the mechanism of action of chemotherapeutic drugs.

    Science (New York, N.Y.)·2010
    Same author

    Cystoisospora belli: in vitro multiplication in mammalian cells.

    Experimental parasitology·2006
    Same author

    Immunological comparison of 124 isolates of Toxoplasma gondii.

    Parasitology research·2003
    Same author

    Determination of the genera of cyst-forming coccidia.

    Parasitology research·2003
    Same author

    Immunocompromise-dependent infection rather than opportunistic infection.

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2001

    Area of Science:

    • Immunology
    • Parasitology
    • Infectious Diseases

    Background:

    • Immunity to the intracellular protozoan Toxoplasma gondii in mice is characterized as premunition.
    • Sulfadiazine chemoprophylaxis typically allows mice to develop immunity against virulent T. gondii.
    • Nude (nu/nu) mice, lacking a thymus, are a model for studying T-cell dependent immunity.

    Purpose of the Study:

    • To investigate the role of cellular immunity in the development of resistance to Toxoplasma gondii in mice.
    • To determine if thymus-derived cells are essential for acquiring immunity during drug prophylaxis.
    • To differentiate the contribution of cellular immunity versus antibody-mediated immunity in T. gondii infection.

    Main Methods:

    • Mice were treated with sulfadiazine to prevent acute toxoplasmosis.
    • Nude mice were compared to their hirsute littermates regarding their ability to develop immunity.
    • Nude mice received intraperitoneal injections of thymus cells, bone marrow cells, or specific antibodies.
    • Immunity development was assessed during and after the drug treatment period.

    Main Results:

    • Nude mice failed to develop immunity to T. gondii during sulfadiazine therapy, unlike their hirsute littermates.
    • Intraperitoneal injection of thymus cells restored the ability of nude mice to develop immunity.
    • Administration of bone marrow cells or high-titered specific antibody did not confer lasting immunity after drug withdrawal.

    Conclusions:

    • Active cellular immunity, likely thymus-dependent, is crucial for developing immunity to Toxoplasma gondii in mice.
    • Antibody-mediated immunity appears to play a less certain or secondary role in this type of immunity.
    • Thymus cell transplantation can restore the capacity for developing cellular immunity in immunodeficient mice.

    Related Experiment Videos