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Morphological and hormonal changes after superovulation in cows treated with Neutra-PMSG

Z Boryczko1, H Bostedt, Z Gajewski

  • 1Department of Animal Reproduction, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.

Archivum Veterinarium Polonicum
|January 1, 1994
PubMed
Summary

Administering Neutra-PMSG 108 hours after pregnant mare serum gonadotropin (PMSG) reduces non-ovulated follicles. This timing optimizes superovulation efficiency without affecting mineral levels in cows.

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Area of Science:

  • Reproductive biology
  • Veterinary endocrinology
  • Animal science

Background:

  • Superovulation is a key technique in assisted reproduction for increasing ovulation rates.
  • Pregnant mare serum gonadotropin (PMSG) is commonly used to induce superovulation.
  • Controlling the timing of PMSG neutralization is crucial for optimizing superovulation outcomes.

Purpose of the Study:

  • To investigate the effects of timing PMSG neutralization on superovulation in cows.
  • To evaluate the impact of Neutra-PMSG administration on ovarian morphology and hormonal profiles.
  • To assess changes in blood plasma mineral levels (Na, K, Ca, Mg, P) following superovulation protocols.

Main Methods:

  • Superovulation was induced using PMSG in cows.
  • Monoclonal antibodies (Neutra-PMSG) were administered at 72 or 108 hours post-PMSG.

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  • Ovarian morphology, follicle counts, corpora lutea, estradiol, progesterone, and plasma mineral levels were analyzed.

    • Main Results:

    • Neutra-PMSG at 108 hours significantly reduced non-ovulated follicles (>10 mm) compared to controls.
    • Ovulation efficiency (corpora lutea) at 108 hours was comparable to PMSG-only treatment.
    • Early Neutra-PMSG administration (72 hours) completely inhibited superovulation.
    • Estradiol levels decreased significantly after 108-hour Neutra-PMSG injection.
    • Progesterone levels correlated with corpora lutea count, aiding superovulation assessment.
    • Blood plasma levels of Na, K, Ca, Mg, and P remained unaffected by the superovulation protocol.

    Conclusions:

    • Administering Neutra-PMSG 108 hours post-PMSG effectively refines superovulation by reducing unovulated follicles.
    • The 108-hour timing allows for efficient ovulation without compromising reproductive outcomes.
    • Progesterone monitoring is a valuable tool for evaluating superovulation success.
    • Superovulation protocols using PMSG and Neutra-PMSG do not alter essential blood plasma mineral concentrations.