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[Electrophoretic analysis on the proteolytic decomposition of of FSH preparations]
H Khalaf1, U Neumann, M Rimpler
1Institut für Medizinische Chemie der Medizinischen Hochschule Hannover.
DTW. Deutsche Tierarztliche Wochenschrift
|October 1, 1995
Abstract:
We investigated the influence of some proteolytic enzymes being present in the digestive canal (pepsin, trypsin, chymotrypsin) on the digestion of porcine FSH-samples by electrophoresis. Trypsin and Chymotrypsin digested the 18-kDa-protein much faster than pepsin, whereas a 67-kDa-protein was much faster digested by Pepsin than by Trypsin or Chymotrypsin. The overall proteolytic effect of Chymotrypsin is small compared with Pepsin and Trypsin. A subsequent digestion with Pepsin, Trypsin and Chymotrypsin led to a complete proteolysis of all proteins being present in the sample.