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Updated: Aug 10, 2026

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
Published on: December 2, 2010
[Use of the method of lanthanide immunofluorescence analysis for detection of lymphocyte Fc-receptors]
Interaction of lymphocyte Fc-receptors (FcR) with the conjugate, containing immunoglobulin G (IgG) labeled with chelating compound of derivative diethylenetriaminepentaacetic acid (DTPA)-DTPA-Eu, was investigated. For optimal conditions in performing lanthanide immunofluorescence analysis (LIFA) with minimal quantity of lymphocyte, i. e. 2 x 10(6) in ml and concentrate for conjugate 10(-8) - 10(-12) pH was found.
Interaction of lymphocyte Fc-receptors (FcR) with the conjugate, containing immunoglobulin G (IgG) labeled with chelating compound of derivative diethylenetriaminepentaacetic acid (DTPA)-DTPA-Eu, was investigated. For optimal conditions in performing lanthanide immunofluorescence analysis (LIFA) with minimal quantity of lymphocyte, i. e. 2 x 10(6) in ml and concentrate for conjugate 10(-8) - 10(-12) pH was found.

