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Related Experiment Videos

Clarithromycin is inactive against Mycobacterium tuberculosis

C Truffot-Pernot1, N Lounis, J H Grosset

  • 1Faculté Médecine Pitié-Salpêtrière, Paris, France.

Antimicrobial Agents and Chemotherapy
|December 1, 1995
PubMed
Summary

Clarithromycin (CLARI) shows minimal effectiveness against Mycobacterium tuberculosis. High minimum inhibitory concentrations (MICs) in vitro and lack of therapeutic effect in mouse models indicate CLARI is inactive as a single drug therapy.

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Area of Science:

  • Microbiology
  • Pharmacology
  • Infectious Diseases

Background:

  • Tuberculosis remains a significant global health challenge.
  • Novel therapeutic agents are needed to combat Mycobacterium tuberculosis.
  • Clarithromycin (CLARI) is a macrolide antibiotic with potential activity against mycobacteria.

Purpose of the Study:

  • To evaluate the in vitro and in vivo efficacy of clarithromycin (CLARI) against Mycobacterium tuberculosis.
  • To determine the minimum inhibitory concentrations (MICs) of CLARI for M. tuberculosis strains.
  • To assess the therapeutic effect of CLARI in a murine model of tuberculosis.

Main Methods:

  • MICs of CLARI were determined for 12 M. tuberculosis strains using Mueller-Hinton agar supplemented with oleic acid, albumin, dextrose, and catalase.

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  • In vivo efficacy was assessed in two mouse experiments involving prolonged treatment with CLARI.
  • Bactericidal and bacteriostatic effects were evaluated in treated mice.

    • Main Results:

    • The MICs of CLARI for 50% and 90% of M. tuberculosis strains were 64 and >128 µg/ml, respectively.
    • These MICs exceed achievable peak serum and lung tissue concentrations of CLARI in humans.
    • Four to six weeks of CLARI treatment in mice showed no bactericidal or bacteriostatic activity against M. tuberculosis.

    Conclusions:

    • Clarithromycin (CLARI) demonstrates poor in vitro activity against Mycobacterium tuberculosis.
    • CLARI concentrations achieved in vivo are unlikely to be effective against M. tuberculosis.
    • Clarithromycin (CLARI) is considered inactive as a single agent for tuberculosis treatment.