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Hantaan virus persistently infects marmoset B-lymphoblastoid cell line
1Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.
Abstract:
To investigate lymphoid cell Hantavirus (HV) interaction, we examined the replication of the HV strains, Hantaan 76-118 (HTN), SR-11, and B-1 with the B95a, LYM-1, CGM1, JM, Jurkat, MOLT-4, AT(L)5KY, BW5147, L1210, and Vero/E6 cells. HV-growth was determined by immunofluorescence and focus-forming assays. LYM-1 and Vero/E6 supported the growth of all HV strains, and B95a only HTN, while remaining cells did not sustain these strains. This is the first report to indicate the HV-replication in lymphoid cell lines. B95a and LYM-1 cells may be useful for revealing the mechanism of HV-infection in animals.
Insights
This study shows Hantavirus (HV) can replicate in specific lymphoid cell lines, unlike most others tested. LYM-1 and Vero/E6 cells supported all HV strains, offering new models for Hantavirus research.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Hantaviruses (HV) are a significant public health concern, causing severe respiratory and hemorrhagic diseases.
- Understanding HV replication mechanisms in host cells is crucial for developing effective treatments and prevention strategies.
Purpose of the Study:
- To investigate the replication capability of different Hantavirus strains in various lymphoid and non-lymphoid cell lines.
- To identify cell lines that can support Hantavirus replication for further mechanistic studies.
Main Methods:
- Replication of Hantavirus strains (Hantaan 76-118, SR-11, B-1) was assessed in a panel of cell lines, including B95a, LYM-1, CGM1, JM, Jurkat, MOLT-4, AT(L)5KY, BW5147, L1210, and Vero/E6.
- Viral growth was quantified using immunofluorescence and focus-forming assays.
Main Results:
- LYM-1 and Vero/E6 cell lines demonstrated susceptibility to all tested Hantavirus strains.
- B95a cells supported the replication of the Hantaan 76-118 strain but not others.
- The majority of the tested cell lines did not support Hantavirus replication.
Conclusions:
- This study reports for the first time Hantavirus replication in lymphoid cell lines.
- LYM-1 and B95a cell lines show promise as valuable tools for elucidating Hantavirus infection mechanisms in animal models.