Related Experiment Videos
Establishment of a bovine macrophage cell line from a fetal thymus
K Yoshihara1, S Tanaka, Y Mori
1National Institute of Animal Health, Ibaraki, Japan.
Abstract:
A cell line, FBM-17, was established from a fetal bovine thymus and passaged for more than a year. Electron microscopic examination revealed that the cytoplasm contained many phagosomes and a significant amount of rough endoplasmic reticula. In addition, FBM-17 cells showed non-specific esterase, phagocytosis and surface expression of Fc receptors for IgG and classes I and II MHC antigens. These observations confirmed that this cell line had the morphologic and functional features as a macrophage. MHC expression markedly increased by treatment with recombinant bovine gamma-interferon, showing the cell line to be sensitive to bovine gamma-interferon and functional. These present results suggest that the FBM-17 is a functional bovine macrophage cell line. This line gamma-interferon, showing the cell line to be useful for clarification of roles of macrophages in immunoregulation in cattle.
Insights
A new bovine macrophage cell line, FBM-17, was developed from fetal bovine thymus. This functional cell line aids research into cattle immunoregulation and macrophage roles.
Area of Science:
- Veterinary Immunology
- Cell Biology
- Bovine Virology
Background:
- Macrophages are crucial immune cells in cattle, but functional cell lines are limited.
- Understanding macrophage function is key to improving cattle health and disease resistance.
Purpose of the Study:
- To establish and characterize a novel fetal bovine thymus-derived macrophage cell line (FBM-17).
- To confirm the morphologic and functional characteristics of the FBM-17 cell line as a macrophage.
- To evaluate the cell line's responsiveness to bovine gamma-interferon for potential use in immunoregulation studies.
Main Methods:
- Cell line establishment from fetal bovine thymus.
- Electron microscopy for ultrastructural analysis.
- Functional assays including esterase activity, phagocytosis, and Fc receptor expression.
- Major histocompatibility complex (MHC) antigen expression analysis before and after stimulation with recombinant bovine gamma-interferon.
Main Results:
- The FBM-17 cell line exhibited phagosomes and abundant rough endoplasmic reticulum, indicative of macrophage morphology.
- FBM-17 cells demonstrated non-specific esterase activity, phagocytosis, and Fc receptor expression.
- Class I and II MHC antigen expression was confirmed on FBM-17 cells.
- MHC expression significantly increased upon treatment with bovine gamma-interferon, confirming cell line functionality and sensitivity.
Conclusions:
- The FBM-17 cell line possesses key morphologic and functional characteristics of bovine macrophages.
- The cell line is sensitive to bovine gamma-interferon, indicating its utility in immunological research.
- FBM-17 serves as a valuable tool for investigating the roles of macrophages in cattle immunoregulation.