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Regulation of glucose transport in the NIH 3T3 L1 preadipocyte cell line by TCDD
1Department of Environmental Toxicology, University of California-Davis 95620, USA.
Environmental Health Perspectives
|May 1, 1994
Abstract:
This study examined the changes in cellular glucose uptake induced by 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) as measured by quantification of intracellular radioactivity in the NIH 3T3 L1 preadipocyte cell line after a 30-minute incubation with the non-metabolizable radioactive analogue of glucose, 3-O-methyl-D-[1-3H] glucose. Treatment of differentiated NIH 3T3 L1 cells with TCDD produced a time- and dose-dependent decrease in the cellular uptake of glucose. Treatment of cells for 3 hr with 10(-8) M TCDD significantly reduced glucose uptake to about 10% of control values (p