Related Experiment Video
Updated: Jul 27, 2026

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
A general database for DNA sequence changes induced by mutagenesis of several bacterial and mammalian genes
1Department of Therapeutic Radiology, Yale University School of Medicine, New Have, CT 06520-8040, USA.
Abstract:
This electronic database is a collection of 225 sets of data on mutations in more than twenty-three thousand mutants (October, 1995) in eleven bacterial genes, five mammalian genes and one gene in yeast cells. Each dataset consists of the changes in DNA sequence in the mutants, typically tens to hundreds, induced by mutagenesis of a particular cell line under specific conditions. The database is available on the Internet and on diskettes, and is periodically updated. Researchers are invited to submit additional data. A data entry program, MUTSIN, is available that diagrams each mutation on the computer screen as entered and alerts the user to any inconsistency between the entry and the wild type gene sequence.
Related Concept Videos
Mutations
Mutations
In vitro Mutagenesis
Mutations
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Mutations in Microorganisms
Spontaneous and Induced Mutations

