Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Are 5S ribosomal RNA primary transcripts edited?

M Szymanski1, M Barciszewska, V A Erdmann

  • 1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Biochemistry and Molecular Biology International
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modification of χ_{c1}(3872) and ψ(2S) Production in pPb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2024
Same author

Amplitude Analysis of the B^{0}→K^{*0}μ^{+}μ^{-} Decay.

Physical review letters·2024
Same author

Fraction of χ_{c} Decays in Prompt J/ψ Production Measured in pPb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2024
Same author

Enhanced Production of Λ_{b}^{0} Baryons in High-Multiplicity pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2024
Same author

Observation of Cabibbo-Suppressed Two-Body Hadronic Decays and Precision Mass Measurement of the Ω_{c}^{0} Baryon.

Physical review letters·2024
Same author

Improved Measurement of CP Violation Parameters in B_{s}^{0}→J/ψK^{+}K^{-} Decays in the Vicinity of the ϕ(1020) Resonance.

Physical review letters·2024
Same journal

Stability of assembled epitopes on human chorionic gonadotropin to covalent modifications.

Biochemistry and molecular biology international·2009
Same journal

Expression or possession of catalase gene does not alter the capacity of M. smegmatis to survive within human macrophages.

Biochemistry and molecular biology international·2009
Same journal

Quantification of epididymal retinoic acid-binding protein (ERABP) mRNA in different anatomical regions of the adult rat epididymis.

Biochemistry and molecular biology international·2009
Same journal

Efficient production of active TNF-alpha by albumin signal peptide.

Biochemistry and molecular biology international·2009
Same journal

The priming effects of some human interleukins on the production of leukotrienes by calcium ionophore stimulated leukocytes.

Biochemistry and molecular biology international·2009
Same journal

Age- and Tissue-specific regulation of chicken inorganic pyrophosphatase.

Biochemistry and molecular biology international·2009
See all related articles

Plant 5S ribosomal RNA (rRNA) gene sequences show differences from mature 5S rRNAs, suggesting potential editing and splicing in their maturation process. These variations, including insertions resembling tRNA introns, hint at complex RNA processing in plants.

Area of Science:

  • Molecular Biology
  • Plant Genetics
  • RNA Processing

Background:

  • Plant 5S ribosomal RNA (rRNA) genes are crucial for protein synthesis.
  • Comparative analysis reveals discrepancies between 5S rRNA gene sequences and mature 5S rRNAs.
  • Understanding these differences is key to elucidating RNA maturation pathways.

Purpose of the Study:

  • To investigate the nature and origin of discrepancies between plant 5S rRNA gene sequences and mature 5S rRNAs.
  • To explore potential mechanisms involved in the processing of 5S rRNA transcripts.

Main Methods:

  • Comparative nucleotide sequence analysis of plant 5S rRNA genes and mature 5S rRNAs.
  • Detailed inspection of sequence differences, including insertions and deletions.
  • Structural analysis of identified insertions to assess potential secondary structures and homologies.

Related Experiment Videos

Main Results:

  • Numerous discrepancies were identified between 5S rRNA gene sequences and mature 5S rRNAs.
  • These differences primarily stem from single nucleotide substitutions, insertions, and deletions.
  • Two longer insertions were found, one 79 nucleotides long, exhibiting potential tRNA-like structure and homology to plant tRNAGly.

Conclusions:

  • The observed sequence variations and structural features of insertions suggest that editing and splicing mechanisms may be involved in the maturation of certain 5S rDNA transcripts.
  • This implies a more complex processing pathway for plant 5S rRNA than previously understood.
  • Further research is warranted to confirm the involvement of these mechanisms in 5S rRNA biogenesis.