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Cloning and sequence analysis of the invertase gene INV 1 from the yeast Pichia anomala
J A Pérez1, J Rodríguez, L Rodríguez
1Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, E-38071 La Laguna, Tenerife, Spain.
Abstract:
A genomic library from the yeast Pichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant of Saccharomyces cerevisiae. The cloned gene, INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also with Kluyveromyces marxianus inulinase, a yeast beta-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.