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Is heparinized plasma suitable for use in routine biochemistry?
J G Donnelly1, S J Soldin, D A Nealon
1Institute for Biomolecular Studies, Catholic University of America, Washington, DC, USA.
Summary
Lithium heparin plasma and serum yield statistically different biochemical test results, but these are not medically significant. Ensure complete filling of heparin tubes to avoid variations in serial testing.
Area of Science:
- Clinical Biochemistry
- Laboratory Medicine
Background:
- Serum and heparinized plasma are common specimen types for biochemical analysis.
- Differences in results between specimen types can impact diagnostic accuracy.
Purpose of the Study:
- To compare biochemical analysis results from lithium heparin plasma and serum.
- To evaluate the impact of lithium heparin concentration on test results.
Main Methods:
- Analyzed 27 biochemical parameters using a Kodak Ektachem 500 analyzer.
- Compared results from lithium heparin plasma and serum.
- Investigated effects of increased lithium heparin concentrations.
Main Results:
- Statistical differences (P < .001) observed in 16 of 27 tests between plasma and serum.
- No medically significant differences were found between plasma and serum results.
- Elevated lithium heparin concentrations caused changes in alanine aminotransferase, amylase, aspartate aminotransferase, lipase, and potassium.
Conclusions:
- Lithium heparin can be used in routine biochemistry to reduce turnaround time.
- Complete filling of heparin anticoagulant tubes is crucial to prevent spurious variations in serial specimen analysis.