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A simple chronic microinjection system for use with chemitrodes

H E Criswell

    Pharmacology, Biochemistry, and Behavior
    |February 1, 1977
    PubMed
    Summary
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    A new, inexpensive microinjection system attaches directly to a Mercury commutator, simplifying chronic chemical stimulation in freely moving animals. This design avoids complex swivel joints for simultaneous recording and stimulation.

    Area of Science:

    • Neuroscience
    • Biomedical Engineering
    • Surgical Instrumentation

    Background:

    • Chronic microinjection in freely moving animals typically requires complex and cumbersome swivel joints.
    • Existing systems can be expensive and difficult to integrate with simultaneous electrophysiological recording or stimulation.

    Purpose of the Study:

    • To describe an inexpensive, electrically operated microinjection system for chronic use in freely moving animals.
    • To enable simultaneous chemical stimulation with electrical stimulation or recording.

    Main Methods:

    • Developed a compact, electrically operated microinjection system designed for direct mounting onto the animal side of a standard Mercury commutator.
    • Integrated the microinjection system with a chemitrode for chemical delivery and a 4-channel Mercury commutator.

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    Main Results:

    • The system is small and inexpensive, eliminating the need for a traditional swivel joint.
    • Successful simultaneous electrical stimulation/recording and chemical stimulation via the chemitrode was achieved.

    Conclusions:

    • This novel microinjection system offers a simplified and cost-effective solution for chronic in vivo chemical stimulation.
    • The design facilitates integrated neurophysiological experiments in freely moving subjects.