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Is aging related to cell signalling alterations in amphibian oocytes?
T M Fonovich de Schroeder1, A M de D'Angelo
1Libiquima, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Neuquén, Argentina.
Abstract:
The storage of Bufo arenarum oocytes decreased their ability to become fertilized "in vitro". The stimulation with carbachol of "young oocytes" showed a persistent hydrolysis with phosphatidylinositol 4,5 diphosphate (PIP2) while in "aged oocytes" both phosphatidylinositol 4 phosphate (PIP) and PIP2 were hydrolyzed at a non-significant level. These results and the lower 32P-phosphoinositide levels found in "aged oocytes" at time of stimulation agree with diminished phosphoinositide kinase and phospholipase C activities, as the consequence of a non-specific phosphoinositide hydrolysis probably occurring during storage.