Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Force production by depolymerizing microtubules: load-velocity curves and run-pause statistics

C S Peskin1, G F Oster

  • 1Courant Institute of Mathematical Sciences, New York, New York 10012, USA.

Biophysical Journal
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Influence of Look-Ahead on the Error Rate of Transcription.

Mathematical modelling of natural phenomena·2011
Same author

Climate change and the integrity of science.

Science (New York, N.Y.)·2010
Same author

Numerical simulation and experimental validation of blood flow in arteries with structured-tree outflow conditions.

Annals of biomedical engineering·2001
Same author

Measurements of mechanical properties of the blastula wall reveal which hypothesized mechanisms of primary invagination are physically plausible in the sea urchin Strongylocentrotus purpuratus.

Developmental biology·1999
Same author

Recovery of cable properties through active and passive modeling of subthreshold membrane responses from laterodorsal tegmental neurons.

Journal of neurophysiology·1998
Same author

Simulating the role of microtubules in depolymerization-driven transport: a Monte Carlo approach.

Biophysical journal·1998
Same journal

Heterogeneous binding of SARS-CoV2 fusion peptide on complex cellular membranes enhances its fusogenicity.

Biophysical journal·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
See all related articles

Microtubule depolymerization drives chromosome transport. A new model explains how ATP enhances this minus-end transport, even though ATP powers kinesin motors in the opposite direction.

Area of Science:

  • Cell Biology
  • Biophysics
  • Molecular Motors

Background:

  • Microtubule depolymerization is known to drive minus-end-directed chromosome transport during mitosis.
  • In vitro experiments show a paradoxical enhancement of this transport by ATP, which normally fuels kinesin motors for plus-end movement.

Purpose of the Study:

  • To develop a mathematical model explaining the ATP-enhanced, depolymerization-driven, minus-end-directed transport of kinesin-coated microspheres.
  • To elucidate the mechanism behind the counterintuitive effect of ATP on microtubule-based transport.

Main Methods:

  • Development of a mathematical model incorporating microsphere-facilitated microtubule depolymerization.
  • Derivation of the force-velocity relationship for the modeled system.
  • Simulation of the stochastic process of microsphere transport and analysis of trajectory statistics.

Related Experiment Videos

Main Results:

  • The model predicts that a microsphere at the microtubule plus end enhances depolymerization, thereby promoting minus-end transport.
  • A unique force-velocity curve was derived, showing maximal velocity at a positive load, not zero load.
  • Simulated trajectories exhibited characteristic runs and pauses, with statistics that allowed parameter determination.

Conclusions:

  • The mathematical model successfully explains the paradoxical enhancement of minus-end-directed transport by ATP.
  • The findings highlight a novel mechanism where motor proteins and depolymerization dynamics interact to regulate intracellular transport.
  • The model's predictive power and ability to determine parameters from simulated trajectories offer insights into motor protein behavior.