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A comprehensive genetic map of the mouse genome
W F Dietrich1, J Miller, R Steen
1Whitehead/MIT Center for Genome Research, Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Nature
|March 14, 1996
Summary
Researchers created a dense genetic map for laboratory mice using over 7,000 markers. This mouse genome map aids in genetic studies and understanding complex traits.
Area of Science:
- Genomics
- Mammalian Genetics
Background:
- Dense genetic linkage maps are crucial for various genomic studies.
- These maps facilitate positional cloning, genetic dissection, and evolutionary comparisons.
Purpose of the Study:
- To develop a comprehensive, high-density genetic linkage map for the laboratory mouse.
- To provide a foundational resource for mouse genomics research.
Main Methods:
- Integration of simple sequence length polymorphisms (SSLP) and restriction fragment length polymorphisms (RFLP).
- Utilized a large number of genetic markers for high-resolution mapping.
Main Results:
- A genetic map comprising 7,377 markers (6,580 SSLPs and 797 RFLPs).
- Achieved an average marker spacing of approximately 0.2 centimorgans (400 kilobases).
Conclusions:
- The finalized mouse genetic map is a valuable resource for diverse genetic and genomic research.
- This map enhances the study of monogenic and polygenic traits, physical mapping, and strain construction.