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Updated: Aug 8, 2026

Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
Response of extremely hypoxic cells to hyperthermia: survival and oxygen enhancement ratios
Abstract:
Chinese hamster V79 cells and mouse EMT6 cells were made extremely hypoxic with a chamber technique that relies upon gas exchange rather than respiration-assisted oxygen depletion. Cells in these chambers were treated with heat (43degrees C) or heat plus x-irradiation, and colony-forming ability was determined. Hyperthermia: (a) kills exponentially growing V79 cells more efficiently than plateau-phase cells; (b) kills euoxic and extremely hypoxic cells equally; and (c) radiosensitizes euoxic and extremely hypoxic cells to the same degree, leaving the oxygen enhancement ratio unchanged.
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