Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A binding factor for interleukin 2 mRNA

J Hua1, V Paetkau

  • 1Department of Biochemistry, Medical Sciences Building, University of Alberta, Edmonton, Canada.

Nucleic Acids Research
|March 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A case of hereditary transthyretin amyloidosis cardiomyopathy complicated with monoclonal gammopathy of undetermined significance].

Zhonghua xin xue guan bing za zhi·2026
Same author

[Clinical characteristics and prognosis analysis of patients with IgG4-related hepatobiliary-pancreatic disease].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024
Same author

[Prospective association study of sleep status and risk of coronary heart disease in adults in Suzhou].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

Adherence to guidelines and central-line-associated bloodstream infection occurrence during insertion and maintenance of intravascular catheters: evidence from 20 tertiary hospitals.

The Journal of hospital infection·2024
Same author

[Haploidentical donor peripheral blood stem cell transplantation using third-party cord blood compared with matched unrelated donor transplantation for patients with hematologic malignancies].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2024
Same author

[Erratum to "Risk factors analysis and establishment of predictive nomogram of extranodal B-cell lymphoma of mucosal-associated lymphoid tissue" [Cancer Radiother 27 (2023) 126-135]].

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2023
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

A novel protein factor in Jurkat cells binds to interleukin 2 mRNA, specifically in the 3' untranslated region. This binding protects the mRNA from degradation by an endoribonuclease.

Area of Science:

  • Molecular Biology
  • Immunology
  • Cell Biology

Background:

  • Jurkat cells are a human T lymphocyte line known for producing interleukin 2.
  • Interleukin 2 (IL-2) production is a critical process in T cell activation and immune response.
  • Regulation of IL-2 mRNA stability is crucial for controlling IL-2 secretion.

Purpose of the Study:

  • To identify and characterize factors within Jurkat cells that bind to interleukin 2 mRNA.
  • To determine the specific binding site and properties of the identified mRNA-binding factor.
  • To investigate the functional role of this factor in regulating IL-2 mRNA stability.

Main Methods:

  • Gel electrophoresis to detect mRNA-protein complex formation.
  • UV-induced cross-linking and protease sensitivity assays to characterize the binding factor.

Related Experiment Videos

  • RNasin-resistant endoribonuclease assays to assess mRNA protection.

    • Main Results:

    • A sequence-specific binding factor for interleukin 2 mRNA was identified in Jurkat cells.
    • The binding site is located in the 3'-non-coding region of the mRNA, near an AU-rich sequence.
    • The factor, likely a protein of 50-60 kDa, protects IL-2 mRNA from endoribonuclease degradation.
    • This binding is distinct from known AU-binding factors.

    Conclusions:

    • A novel protein factor regulates interleukin 2 mRNA stability in Jurkat cells.
    • This factor binds to a specific site in the 3'-UTR, preventing mRNA degradation.
    • The findings provide insights into the post-transcriptional regulation of IL-2 production.