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Related Experiment Videos

Simple and sensitive enzyme-linked immunosorbent assay for ivermectin

Y Mitsui1, H Tanimori, T Kitagawa

  • 1Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.

The American Journal of Tropical Medicine and Hygiene
|March 1, 1996
PubMed
Summary

A new enzyme-linked immunosorbent assay (ELISA) accurately measures ivermectin (IVM) in biological fluids. This sensitive method allows for easy determination of ivermectin serum levels in animal studies.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Veterinary Medicine

Background:

  • Accurate quantification of ivermectin (IVM) in biological samples is crucial for pharmacokinetic and efficacy studies.
  • Existing methods may lack the sensitivity or reproducibility required for precise IVM determination.
  • Development of a robust assay is needed to support research involving ivermectin.

Purpose of the Study:

  • To develop and validate a sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for quantifying ivermectin (IVM) in biological fluids.
  • To establish the assay's performance characteristics, including sensitivity, precision, and specificity.
  • To demonstrate the utility of the developed ELISA in a relevant animal model.

Main Methods:

  • A competitive ELISA format was employed using an ivermectin-biotin conjugate and specific antibodies.

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  • Antibodies were generated in rabbits against an ivermectin-bovine serum albumin conjugate.
  • The assay involved simultaneous incubation of sample IVM and conjugate, followed by detection using avidin-peroxidase.

    • Main Results:

    • The developed ELISA demonstrated high sensitivity with a limit of detection of 0.1 ng/ml and a working range of 0.3-10 ng/ml.
    • The assay exhibited excellent reproducibility, with a coefficient of variation less than 10%.
    • Negligible cross-reactivity was observed with other anthelmintic drugs, indicating high specificity.

    Conclusions:

    • A sensitive, reproducible, and specific competitive ELISA for ivermectin determination in biological fluids has been successfully developed.
    • This assay enables accurate measurement of ivermectin serum levels in animal models, facilitating pharmacokinetic research.
    • The established ELISA provides a valuable tool for research requiring precise quantification of ivermectin concentrations.