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Related Experiment Videos

p53 expression is rare in cutaneous melanomas

M C Saenz-Santamaría1, N S McNutt, J K Bogdany

  • 1Department of Dermatology, New York Hospital-Cornell Medical Center, New York 10021, USA.

The American Journal of Dermatopathology
|August 1, 1995
PubMed
Summary

p53 protein alterations are rare in cutaneous melanoma, but overexpression may occur in highly proliferative melanomas. This study investigated p53 expression in melanoma tissues using immunohistochemistry.

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Area of Science:

  • Oncology
  • Dermatology
  • Molecular Biology

Background:

  • Alterations in the tumor-suppressor gene p53 are common in human malignancies.
  • The role of p53 in cutaneous melanoma pathogenesis is controversial.
  • p53 protein accumulates in cells with missense mutations, allowing immunohistochemical detection.

Purpose of the Study:

  • To investigate the expression of p53 protein in cutaneous melanomas and melanocytic nevi.
  • To determine if p53 abnormalities correlate with cellular proliferation in melanoma.

Main Methods:

  • Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections.
  • A panel of three anti-p53 monoclonal antibodies (mAbs) and a Ki-67 mAb (MIB-1) were used.
  • Tissues examined included primary invasive melanomas, melanoma metastases, and melanocytic nevi.

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Main Results:

  • p53 was not detected in normal epidermal melanocytes or nevus cells.
  • One primary invasive melanoma with high proliferation (>50% Ki-67) showed diffuse nuclear p53 labeling with all three mAbs.
  • Abnormal p53 expression was rare in cutaneous melanomas.

Conclusions:

  • p53 abnormalities are infrequent in cutaneous melanoma.
  • Overexpression of p53 protein may occur in a subset of melanomas characterized by high cellular proliferation.