Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An automated, specific, spectrophotometric method for measuring ascorbic acid in plasma (EFTSA)

I F Benzie1

  • 1Department of Health Sciences, Hong Kong Polytechnic University, Hong Kong.

Clinical Biochemistry
|April 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous automated measurement of total 'antioxidant' (reducing) capacity and ascorbic acid concentration.

Redox report : communications in free radical research·2016
Same author

Uric acid: friend or foe?

Redox report : communications in free radical research·2016
Same author

Assessing Age-Related Changes in Antioxidant Status : The FRASC Assay for Total Antioxidant Power and Ascorbic Acid Concentration in Biological Fluids.

Methods in molecular medicine·2012
Same author

Opposing responses of apoptosis and autophagy to moderate compression in skeletal muscle.

Acta physiologica (Oxford, England)·2010
Same author

"Antioxidant" (reducing) efficiency of ascorbate in plasma is not affected by concentration.

The Journal of nutritional biochemistry·2004
Same author

Plasma ascorbic acid: measurement, stability and clinical utility revisited.

Clinical biochemistry·2002

This study shows that the automated enzyme linked, ferric-tripyridyltriazine spectrophotometric assay (EFTSA) is a reliable method for measuring plasma ascorbic acid. The EFTSA method is suitable for routine clinical use.

Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Accurate measurement of plasma ascorbic acid is crucial for assessing nutritional status and diagnosing related conditions.
  • Existing methods may have limitations in terms of automation, speed, or specificity.

Purpose of the Study:

  • To evaluate the performance of an automated enzyme linked, ferric-tripyridyltriazine spectrophotometric assay (EFTSA) for quantifying plasma ascorbic acid.
  • To determine the suitability of EFTSA for routine clinical laboratory use.

Main Methods:

  • The study assessed reaction kinetics, dose-response relationships, recovery of added ascorbic acid, specificity, and precision.
  • Experiments were conducted using aqueous ascorbic acid solutions and plasma samples on a Cobas Fara centrifugal analyzer.

Main Results:

Related Experiment Videos

  • The EFTSA method demonstrated linearity up to 400 micromol/L with within-run coefficients of variation (CVs) below 5.5% across various concentrations.
  • Recovery of added ascorbic acid ranged from 99-105%, indicating high accuracy.
  • The assay showed rapid reaction kinetics and no significant interference from other native antioxidants or dehydroascorbic acid within a specific time window.

Conclusions:

  • The automated enzyme linked, ferric-tripyridyltriazine spectrophotometric assay (EFTSA) is a precise and accurate method for plasma ascorbic acid measurement.
  • EFTSA is well-suited for routine clinical application due to its performance characteristics and automation compatibility.