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Related Experiment Videos

Nuclear morphometry during the cell cycle

F Kendall, R Swenson, T Borun

    Science (New York, N.Y.)
    |June 3, 1977
    PubMed
    Summary
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    Nuclear morphology significantly changes throughout the cell cycle in HeLa cells. These alterations occur even during G1 and G2 phases, and during transitions between cell cycle stages.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Genetics

    Background:

    • Understanding nuclear morphology dynamics is crucial for cell cycle regulation.
    • Previous studies have focused on specific cell cycle phases, leaving gaps in knowledge regarding continuous morphological changes.

    Purpose of the Study:

    • To quantitatively analyze nuclear morphology changes during the entire cell cycle.
    • To investigate alterations in geometric and densitometric parameters of the nucleus.

    Main Methods:

    • Utilized automated image analysis with the Quantimet 720-D system.
    • Employed Feulgen staining for DNA visualization in synchronized HeLa cells.
    • Synchronized cells using selective mitotic detachment to ensure cell cycle progression.

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    Main Results:

    • Demonstrated substantial alterations in nuclear morphology across all cell cycle phases.
    • Observed significant changes even during the G1 and G2 phases.
    • Identified notable nuclear shape and density variations during late G1-early S and late S-G2 transitions.

    Conclusions:

    • Nuclear morphology is not static and undergoes continuous, significant changes throughout the cell cycle.
    • These dynamic changes in nuclear parameters are evident even in seemingly quiescent phases like G1 and G2.
    • The study highlights the dynamic nature of the nucleus and its implications for cell cycle progression and function.