[Distribution of the kappa-casein gene alleles in the bison (Bison bonasus) population]

The polymerase chain reaction method, followed by restriction fragment length polymorphism analysis (PCR-RFLP), was used to study the alleles of the kappa-casein gene in a sample of European bison (n = 38). On the basis of the distribution of the restriction sites, a new allele kappa-CnG was revealed in the European bison, in addition to the kappa-CnA allele represented in cattle. The nucleotide sequence of an exon 4 fragment of the kappa-CnG allele was obtained. In the population of European bison alleles kappa-CnA and kappa-CnG occurred at frequencies 0.224 and 0.776, respectively.