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Related Experiment Videos

Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast

W A Wells1, A W Murray

  • 1Department of Physiology, University of California, San Francisco, 94143-0444, USA.

The Journal of Cell Biology
|April 1, 1996
PubMed
Summary

Mini-chromosomes in budding yeast reveal how cells detect chromosome misalignments. Short, unstable mini-chromosomes frequently delay mitosis, highlighting the spindle assembly checkpoint's role in preventing errors.

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The spindle assembly checkpoint (SAC) prevents cell cycle progression with chromosome alignment defects.
  • Understanding SAC mechanisms is crucial for cell division fidelity.

Purpose of the Study:

  • To investigate the impact of mini-chromosomes on the SAC in budding yeast.
  • To elucidate how chromosome misbehavior is detected by the SAC.

Main Methods:

  • Pedigree analysis in budding yeast (Saccharomyces cerevisiae).
  • Observation of cell cycle delays in individual cells.
  • Utilizing mini-chromosomes with varying properties (linear, circular, length, centromere strength).

Main Results:

Related Experiment Videos

  • Short, centromeric linear mini-chromosomes induced frequent mitotic delays due to low segregation fidelity.
  • Circular and longer linear mini-chromosomes showed fewer delays, but these were concentrated in unequal segregation events.
  • Increased copy number of circular mini-chromosomes, via a conditional centromere, significantly raised delay frequency.
  • All observed delays were abolished in mad mutants, confirming the requirement of Mad gene products.

    • Conclusions:

    • Mini-chromosomes effectively probe the sensitivity of the SAC to subtle chromosome segregation errors.
    • The SAC, involving Mad gene products, is essential for detecting and responding to chromosome misbehavior, similar to higher eukaryotes.