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Peroxidase changes in barley induced by ionizing and thermal radiation
N K Sah1, S Pramanik, S S Raychaudhuri
1Department of Botany, R.D. College, Sheikhpura, Munger, India.
International Journal of Radiation Biology
|January 1, 1996
Summary
Both gamma-ray and thermal radiation damage barley seeds, but affect peroxidase activity differently. Gamma-rays increase activity, while thermal radiation decreases it without changing isozyme patterns, suggesting distinct damage pathways.
Area of Science:
- Plant Science
- Radiation Biology
- Biochemistry
Background:
- Barley seeds (IB65) were subjected to thermal and ionizing (gamma-ray) radiation.
- Understanding radiation-induced damage mechanisms is crucial for agricultural applications.
Purpose of the Study:
- To compare the effects of thermal and gamma-ray radiation on barley seed damage.
- To investigate the impact of these radiations on peroxidase activity and isozyme patterns.
Main Methods:
- Induction of damage in barley seeds using thermal and gamma-ray radiation.
- Enzyme assays to measure peroxidase activity.
- Electrophoretic analysis to determine peroxidase isozyme banding patterns.
Main Results:
- Both radiation types caused observable damage to barley seeds, evidenced by reduced seedling height.
- Gamma-rays significantly enhanced peroxidase activity.
- Thermal radiation reduced peroxidase activity but did not alter the number of peroxidase isozymes.
Conclusions:
- The mechanisms of damage induced by thermal and ionizing radiations in barley seeds are distinct.
- Differential effects on peroxidase activity suggest unique cellular responses to each radiation type.