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Updated: Sep 12, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Characterization and inhibition of a cholecystokinin-inactivating serine peptidase
C Rose1, F Vargas, P Facchinetti
1Unité de Neurobiologie et Pharmacologie de l'INSERM, Centre Paul Broca, Paris, France.
Abstract:
A cholecystokinin (CCK)-inactivating peptidase was purified and identified as a membrane-bound isoform of tripeptidyl peptidase II (EC 3.4.14.10), a cytosolic subtilisin-like peptidase of previously unknown functions. The peptidase was found in neurons responding to cholecystokinin, as well as in non-neuronal cells. Butabindide, a potent and specific inhibitor, was designed and shown to protect endogenous cholecystokinin from inactivation and to display pro-satiating effects mediated by the CCKA receptor.
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