Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmin accelerates platelet-dependent prothrombinase formation without activating the platelets

L Liu1, J Freedman, A Hornstein

  • 1Canadian Red Cross Society, Blood Services, Hamilton Ontario, Canada.

British Journal of Haematology
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A phenome-wide association study of cross-disorder genetic liability in youth genetically similar to individuals from European reference populations.

Nature. Mental health·2026
Same author

Durvalumab and tremelimumab plus local partial tumour ablation (radiofrequency ablation or stereotactic radiotherapy) in patients with unresectable liver metastases from metastatic colorectal cancer: results of the EORTC-1560-GITCG multicentre, single-arm phase II study (ILOC).

ESMO open·2025
Same author

Phenome-wide Investigation of Behavioral, Environmental, and Neural Associations with Cross-Disorder Genetic Liability in Youth of European Ancestry.

medRxiv : the preprint server for health sciences·2023
Same author

Changes to country-specific hepatitis A travel vaccination recommendation for UK travellers in 2017-responding to a vaccine shortage in the national context.

Public health·2018
Same author

The impact of new national guidance for the public health management of enteric fever in England.

Public health·2017
Same author

High frequency jet ventilation for motion management during ablation procedures, a narrative review.

Acta anaesthesiologica Scandinavica·2017

Plasmin does not directly activate platelets or prothrombin. However, it potentiates thrombin-induced platelet activation and accelerates prothrombin activation, potentially explaining side effects of thrombolytic therapy.

Area of Science:

  • Biochemistry
  • Hematology
  • Cardiovascular Research

Background:

  • Thrombolytic therapy for myocardial infarction can lead to increased platelet activation and thrombosis.
  • The precise mechanisms underlying these adverse effects require further investigation.

Purpose of the Study:

  • To investigate whether plasmin, a key enzyme in thrombolysis, directly activates platelets and prothrombin.
  • To elucidate plasmin's role in potentiating platelet activation and thrombin generation.

Main Methods:

  • Experiments were conducted using recalcified platelet-rich plasma (RPRP) with varying concentrations of plasmin and inhibitors.
  • Platelet activation markers (GMP-140, GP IIb-IIIa, GPIb) and prothrombin activation (prothrombin fragment 1 + 2) were measured.
  • Prothrombin activation was also assessed in washed platelets under specific conditions with alpha-thrombin and factor Xa.

Related Experiment Videos

Main Results:

  • Plasmin alone did not activate platelets or prothrombin in RPRP.
  • Plasmin accelerated platelet activation, secretion, and prothrombin fragment 1 + 2 production in RPRP.
  • Plasmin enhanced alpha-thrombin-induced platelet activation marker expression and potentiated prothrombin activation.

Conclusions:

  • Plasmin does not directly activate platelets or prothrombin.
  • Plasmin potentiates the platelet release reaction and enhances prothrombin activation, likely by increasing factor V availability.
  • These findings suggest a mechanism contributing to thrombotic side effects observed after thrombolytic therapy.