Related Experiment Video
Updated: Aug 17, 2026
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Published on: July 22, 2021
UV-induced photoproducts in human skin explants analysed by TLC and HPLC-radioactivity detection
1Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Abstract:
The objective of this study was to apply the 32P-postlabelling method for the detection of UV-induced dipyrimidine photoadducts in human skin explants. [32P]TLC and [32P]HPLC analysis were used. These techniques allow detection of different combinations of cyclobutane dimers and 6-4 photoproducts in human skin at low doses of irradiation. Detection limit of the present method for some dinucleotide adducts is < 1 adduct per 10(7) normal nucleotides. Reaction of photochemical reversion of the cyclobutane dimers was performed to confirm the nature of the products detected in human skin explants.
