Related Experiment Videos
Intraocular levels of methotrexate after intravenous administration
M D de Smet1, V Stark-Vancs, D R Kohler
1Clinical Immunology Section, National Eye Institute, Bethesda, MD 20892-1858, USA.
Purpose:
To determine if adequate intraocular levels of methotrexate are achieved after intravenous administration.
Methods:
After intravenous administration, methotrexate levels were determined in the serum, the anterior chamber, and the cerebrospinal fluids of a patient with recurrent ocular lymphoma. A fluorescence polarization immunoassay was used to make the determinations.
Results:
At seven hours into a 24-hour intravenous infusion, methotrexate was at cytotoxic level in all samples. At 74 hours, cytotoxic levels were present only in the aqueous humor.
Conclusion:
Sustained cytotoxic ocular methotrexate levels are achievable after systemic administration.