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Use of proZZ-obelin fusion protein in bioluminescent immunoassay
L A Frank1, V A Illarionova, E S Vysotski
1Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russia.
Biochemical and Biophysical Research Communications
|February 15, 1996
Abstract:
Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coli by recombinant DNA techniques. The proZZ-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 X 10(15) photons per mg of protein.