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Microinjection of fluorescence dye in a plant cell and its intercellular translocation using a multi-channel
1Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Biochimica Et Biophysica Acta
|February 9, 1996
Abstract:
Lucifer yellow CH dye (LY) was electrophoretically injected into a cell of a cultured tobacco (BY-2) using a multi-channel microelectrode (MME). Pulsing electricx potential (pulse width:90 ms, pulse interval: 80 ms) was applied between the potential-applying (PA) electrodes of both MMESs . The PA-electrode positioned in the LY-bearing cell was set negative against the other PA-electrode. When the pulse height was 340 V, intercellular translocation of LY from the LY-bearing cell to the neighboring cell was observed within 10 s.