Purification and characterization of a pod lectin from Great Northern bean, Phaseolus vulgaris L

The pods of the Great Northern bean plant contain a lectin (GNpL) that highly resembles seed lectins (GNLs) of the same plant. Purification of GNpL from pod extracts was achieved by ion-exchange chromatographies on CM- and DEAE-celluloses and gel filtration chromatography on Sephacryl S-300 HR. GNpL has a similar SDS-PAGE pattern to that of GNLs. GNpL and GNLs yield three subunits though each GNpL subunit is 0.5 kDa smaller than the corresponding GNLs subunit (GNpL; pod-alpha-subunit of 34.0 kDa, pod-beta-subunit of 36.5 kDa, and pod-gamma-subunit of 38.5 kDa). GNpL and GNLs display indistinguishable carbohydrate specificities and have similar amino acid compositions. Pod-alpha-subunit cross-reacts with antibodies against GNLs on western blotting. On the other hand, the N-terminal amino acid sequence of pod-alpha-subunit suggests that GNpL is a distinct gene product from those of GNLs genes although they are shown to be homologous proteins.