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Related Experiment Videos

Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter

K Miyamoto1, T Shiraga, K Morita

  • 1Department of Clinical Nutrition, School of Medicine, Tokushima University, Japan.

Biochimica Et Biophysica Acta
|February 7, 1996
PubMed
Summary

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Researchers identified the rat PepT1 transporter, crucial for peptide absorption in the small intestine. Its gene expression significantly changes during development, impacting nutrient uptake.

Area of Science:

  • Molecular Biology
  • Genetics
  • Physiology

Background:

  • The peptide transporter PepT1 (Oligopeptide Transporter 1) plays a vital role in the absorption of di- and tripeptides in the small intestine.
  • Understanding the molecular characteristics and regulation of PepT1 is crucial for comprehending nutrient transport and drug delivery.

Purpose of the Study:

  • To isolate and characterize the complementary DNA (cDNA) encoding the rat PepT1 transporter.
  • To investigate the expression patterns of rat PepT1 mRNA in different tissues and during postnatal development.

Main Methods:

  • Isolation of rat PepT1 cDNA clones from a jejunal library using cross-hybridization with a rabbit PepT1 cDNA probe.
  • Sequence analysis to determine the amino acid composition and identity with other species' PepT1.

Related Experiment Videos

  • Northern blot analysis to detect and quantify rat PepT1 mRNA levels in various tissues and developmental stages.

    • Main Results:

    • Rat PepT1 cDNA was successfully isolated, revealing a protein of 710 amino acids.
    • Rat PepT1 shares significant amino acid sequence identity with rabbit (77%) and human (83%) PepT1.
    • Rat PepT1 mRNA was detected in the small intestine and kidney, with highest levels in 4-day-old rats, decreasing to adult levels by day 28.

    Conclusions:

    • The rat PepT1 transporter has been molecularly characterized.
    • PepT1 gene expression in rats undergoes significant developmental regulation, particularly in the small intestine.
    • These findings provide insights into the ontogeny of peptide absorption and potential implications for drug development.