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Related Experiment Videos

Activated B cells express functional Fas ligand

M Hahne1, T Renno, M Schroeter

  • 1Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.

European Journal of Immunology
|March 1, 1996
PubMed
Summary

B lymphocytes express functional Fas ligand (FasL), a protein that triggers apoptosis. This discovery suggests the Fas system plays a role in regulating B cell numbers and maintaining immune homeostasis.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Fas ligand (FasL) is a key mediator of apoptosis, typically expressed by cytotoxic immune cells like T cells and NK cells.
  • Binding of FasL to its receptor (Fas) initiates programmed cell death, crucial for immune regulation.

Purpose of the Study:

  • To investigate the expression and function of FasL in B lymphocytes.
  • To determine if B cells contribute to immune regulation through the Fas system.

Main Methods:

  • Flow cytometry to detect surface FasL expression on B cells.
  • Western blot and RT-PCR to confirm FasL expression at the molecular level.
  • Functional assays using B cells from wild-type and gld mutant mice to assess cytotoxic activity.

Main Results:

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  • B lymphocytes express FasL on their surface upon stimulation with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate/ionomycin.
  • Confirmed FasL expression in activated B cells using molecular techniques.
  • LPS-activated B cells from wild-type mice, but not gld mutant mice, demonstrated the ability to kill Fas-sensitive target cells, confirming functional FasL.

Conclusions:

  • B lymphocytes can express functional FasL, expanding the known cell types involved in Fas-mediated apoptosis.
  • The Fas system, through B cell-expressed FasL, may play a significant role in controlling B cell homeostasis and immune regulation.