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Related Experiment Videos

Electron probe microanalysis: its present, its future

C P Lechene

    The American Journal of Physiology
    |May 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Electron probe microanalysis (EPM) enables elemental ultramicroanalysis of tiny biological samples. This technique allows for precise elemental quantitation in volumes less than 1 micronm3, advancing microphysiology research.

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    Area of Science:

    • Analytical Chemistry
    • Materials Science
    • Biophysics

    Background:

    • Electron probe microanalysis (EPM) is an established technique for elemental analysis.
    • Existing ultramicroanalytical methods have limitations in sample volume and elemental range.
    • Biological applications of EPM require specialized sample preparation techniques.

    Purpose of the Study:

    • To highlight the capabilities of Electron Probe Microanalysis (EPM) for biological ultramicroanalysis.
    • To discuss advancements in sample preparation for biological EPM.
    • To explore the potential of EPM in various fields of microphysiology.

    Main Methods:

    • EPM utilizes characteristic X-ray spectrometry excited by an electron beam for elemental analysis.
    • Analysis of sample volumes down to 1 micronm3 (10(-15) liter) and quantitation to 10(-15) g.

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  • Development of methods for analyzing liquid droplets (10(-11) liter) and isolated cells.
  • Application of frozen hydrated tissue manipulation near liquid nitrogen temperature for diffusible element analysis.

    • Main Results:

    • EPM allows analysis of more elements (from carbon upwards) in smaller volumes than other ultramicroanalytical methods.
    • Quantitation of elemental content in isolated cells is achievable.
    • Methods are emerging for analyzing cellular, extracellular, and intercellular content simultaneously.
    • High-resolution localization of organic compounds using tags is becoming feasible.

    Conclusions:

    • EPM offers unprecedented sensitivity and spatial resolution for elemental analysis in biological samples.
    • Advancements in sample preparation are expanding EPM's utility in biology.
    • EPM is poised to significantly benefit microphysiology and related scientific disciplines.