Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of DNA-porphyrin complex

L A Lipscomb1, F X Zhou, S R Presnell

  • 1School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, 30332-0400, USA.

Biochemistry
|March 5, 1996
PubMed
Summary

A copper metalloporphyrin (CuTMPyP4) complex with DNA was studied using X-ray crystallography. The structure reveals the small molecule extrudes a DNA base, destabilizing the helix through steric clashes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Visual analysis of global research hotspots and development trends in dental trauma].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

[Immune-related genes and their determined immune cell microenvironment to predict the prognosis of gastric adenocarcinoma].

Zhonghua yi xue za zhi·2022
Same author

Engineering new limits to magnetostriction through metastability in iron-gallium alloys.

Nature communications·2021
Same author

[Establishment of a nomogram model for predicting lymph node metastasis in patients with cN0 gastric cancer based on combination of preoperative C-reactive protein/albumin ratio].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2019
Same author

[One case report for branchial cleft carcinoma].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2017
Same author

CTC1 increases the radioresistance of human melanoma cells by inhibiting telomere shortening and apoptosis.

International journal of molecular medicine·2014

Area of Science:

  • Structural Biology
  • Biochemistry
  • Molecular Biophysics

Background:

  • DNA-small molecule interactions are crucial for biological processes and drug development.
  • Metalloporphyrins are a class of compounds with diverse biological activities.
  • Understanding the structural basis of DNA-porphyrin complexes provides insights into DNA recognition and modification.

Purpose of the Study:

  • To elucidate the 3D structure of a complex formed between a copper metalloporphyrin (CuTMPyP4) and a DNA hexamer duplex.
  • To investigate the molecular mechanisms by which CuTMPyP4 interacts with and alters DNA structure.
  • To understand how porphyrin binding affects DNA helical stability and base pairing.

Main Methods:

  • X-ray crystallography at 2.4 A resolution.

Related Experiment Videos

  • Structural analysis of the CuTMPyP4-DNA complex.
  • Analysis of intermolecular interactions, including electrostatic and steric forces.

    • Main Results:

    • The X-ray structure revealed CuTMPyP4 intercalated within the DNA duplex, extruding one cytosine base.
    • The DNA formed a distorted helix with only four Watson-Crick base pairs.
    • Extensive electrostatic interactions stabilized the complex, while steric clashes between pyridyl rings and the DNA backbone destabilized the duplex.

    Conclusions:

    • CuTMPyP4 binding induces significant DNA structural distortion, including base extrusion.
    • Steric clashes between the porphyrin and DNA backbone destabilize the DNA helix, reducing the energy barrier for local melting and base extrusion.
    • The findings highlight a novel mechanism of DNA-small molecule interaction driven by electrostatic stabilization and steric destabilization.