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Defects in hemostasis in P-selectin-deficient mice
M Subramaniam1, P S Frenette, S Saffaripour
1Center for Blood Research, Boston, MA 02115, USA.
Blood
|February 15, 1996
Summary
P-selectin plays a crucial role in hemostasis, the process that stops bleeding. Mice lacking P-selectin exhibited prolonged bleeding times and larger hemorrhagic lesions, confirming its importance in blood clotting.
Area of Science:
- Hematology
- Immunology
- Cellular Biology
Background:
- Platelets and leukocytes roll on activated endothelium expressing P-selectin.
- This suggests a potential role for P-selectin in hemostasis.
Purpose of the Study:
- To investigate the role of P-selectin in hemostasis using P-selectin-deficient mice.
- To confirm the contribution of P-selectin to platelet function in vivo.
Main Methods:
- Tail amputation bleeding time assay in P-selectin-deficient mice.
- Induction of a local Shwartzman-like reaction with lipopolysaccharide and tumor necrosis factor-alpha.
- Quantification of hemorrhagic lesions macroscopically and microscopically.
- Measurement of radioactivity in skin using chromium-labeled red blood cells.
Main Results:
- P-selectin-deficient mice showed a 40% prolongation in bleeding time.
- Defective hemostasis and twofold larger hemorrhagic lesions were observed in P-selectin-deficient mice.
- Radioactivity measurements confirmed increased bleeding in deficient mice.
Conclusions:
- P-selectin plays a significant role in hemostasis.
- P-selectin supports platelet rolling, contributing to effective clot formation and bleeding control.