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Keratin expression in discoid lupus erythematosus
D de Berker1, D Dean, I M Leigh
1Department of Dermatology, Churchill Hospital, Oxford, UK.
Experimental Dermatology
|December 1, 1995
Summary
Discoid lupus erythematosus (DLE) involves abnormal keratin distribution in skin cells, with basal cells showing suprabasal markers. This suggests increased proliferation and basement membrane disruption in DLE lesions.
Area of Science:
- Dermatology
- Immunohistology
- Cell Biology
Background:
- Discoid lupus erythematosus (DLE) is a chronic skin condition.
- Understanding keratinocyte and basement membrane changes in DLE is crucial.
Purpose of the Study:
- To investigate keratin distribution and basement membrane zone (BMZ) status in DLE lesions.
- To compare DLE keratin profiles with normal skin.
Main Methods:
- Immunohistological examination of 21 DLE lesions using monoclonal antibodies for specific keratins (K1, K10, K14, K16) and type VII collagen.
- Analysis of keratin distribution in basal, differentiating, and hyperproliferating keratinocytes.
Main Results:
- Keratin distribution in DLE lesions differed significantly from controls.
- Suprabasal keratin markers (LH6, LH8) were found in 95% and 79% of DLE specimens, respectively.
- Reduced K1 expression (59%) and increased K16 expression (33%) were observed, with inverse correlation.
Conclusions:
- Findings support a model of DLE with increased basal compartment proliferation.
- Basement membrane disruption and dermal colloid body formation are associated with altered keratin expression in DLE.