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Updated: May 13, 2026
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins
M S Dixon1, D A Jones, J S Keddie
1Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Cell
|February 9, 1996
Summary
Plant resistance genes like Cf-2 are crucial for pathogen defense. This study isolated tomato Cf-2 genes, revealing their role in recognizing specific avirulence (Avr) genes from C. fulvum, enhancing plant immunity.
Area of Science:
- Plant molecular biology
- Plant-pathogen interactions
- Genetics
Background:
- Plant disease resistance is often mediated by dominant resistance genes.
- These genes' products are hypothesized to detect pathogen-encoded avirulence (Avr) proteins.
- The tomato leaf mold pathogen, *C. fulvum*, serves as a model for studying plant-pathogen recognition.
Purpose of the Study:
- To isolate and characterize the tomato resistance locus Cf-2.
- To understand the molecular basis of Cf-2-mediated resistance to *C. fulvum*.
- To investigate the structural features of Cf-2 proteins and their potential role in plant defense signaling.
Main Methods:
- Positional cloning was employed to isolate the Cf-2 locus from tomato.
- Sequence analysis was performed on the identified Cf-2 genes.
- Comparative analysis was conducted with other known resistance genes, such as Cf-9.
Main Results:
- The Cf-2 locus contains two highly similar genes, each conferring resistance to specific *C. fulvum* strains expressing the corresponding Avr2 gene.
- The Cf-2 genes encode proteins with 38 leucine-rich repeat (LRR) motifs, 20 of which exhibit conserved alternating repeats.
- The C-terminal regions of Cf-2 proteins show homology to the Cf-9 gene product.
Conclusions:
- The identified Cf-2 genes are essential for recognizing *C. fulvum* avirulence factors.
- The structural features of Cf-2 proteins, particularly the LRR motifs, are likely involved in pathogen recognition.
- The conserved C-terminal region may interact with downstream signaling components to activate plant defense mechanisms.
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