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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: May 14, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 28, 2010

Cefaclor serum sickness

¹Department of Medicine, Northwestern University, Chicago, Illinois, USA.

|April 17, 1996

Summary

No abstract available in PubMed .

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