Related Experiment Videos
Crystal structure of a yeast TFIIA/TBP/DNA complex
S Tan1, Y Hunziker, D F Sargent
1Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, Zürich, Switzerland.
Nature
|May 9, 1996
Abstract:
The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-sheet and bridges over the DNA major groove immediately upstream of the TATA box. The four-helix bundle contributes substantially to the surface of the complex available for interaction with additional transcription factors.