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Platelet-activating factor in brain regions after transient ischemia in gerbils
1Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Md., 20892-1262, USA.
Stroke
|March 1, 1996
Summary
Platelet-activating factor (PAF) significantly increased in gerbil brains after ischemia and reperfusion, particularly in the hippocampus, cortex, and thalamus. These early PAF elevations suggest a role in ischemic brain damage.
Area of Science:
- Neuroscience
- Biochemistry
- Pathology
Background:
- Platelet-activating factor (PAF) is implicated in ischemic brain damage based on indirect evidence.
- Direct measurement of PAF in neuronal tissues during ischemia has been lacking.
Purpose of the Study:
- To directly measure regional brain PAF concentrations after ischemia and reperfusion in gerbils.
- To provide direct evidence for PAF's involvement in ischemic brain damage.
Main Methods:
- Transient ischemia (10 minutes) followed by reperfusion (0-96 hours) in gerbils.
- Quantitative analysis of PAF in brain regions using electron-capture negative chemical ionization GC/MS.
Main Results:
- Maximal PAF increases observed after 1 hour of reperfusion: hippocampus (211%), cortex (168%), thalamus (169%).
- No significant PAF changes were detected from 6 to 96 hours post-reperfusion.
Conclusions:
- PAF levels rise in gerbil brains during early stages of reperfusion following ischemia.
- Elevated PAF may contribute to the development and progression of ischemic neuropathology.