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Related Experiment Videos

Cellular crossreactivity. Implications for solid organ transplantation matching

A H Johnson1, H Araujo, T F Tang

  • 1Department of Pediatrics, Georgetown University Medical Center, Washington, DC 20007, USA.

Transplantation
|February 27, 1996
PubMed
Summary
This summary is machine-generated.

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This study reveals T lymphocyte clone (TLC) crossreactivity among DR11, DR13, and DR8 molecules. Identifying these cellular interactions can improve donor matching for retransplant patients, especially in diverse populations.

Area of Science:

  • Immunology
  • Transplantation immunology
  • HLA and disease

Background:

  • Cellular crossreactivity among HLA-DR molecules, particularly DR11 microvariants, poses challenges in donor-recipient matching.
  • Serological matching for HLA-DR11 is insufficient due to unidentifiable microvariants, leading to potential mismatches in retransplantation.

Purpose of the Study:

  • To evaluate the cellular crossreactivity between DR11, DR13, and DR8 molecules.
  • To explore the implications of DR11 microvariant crossreactivity for retransplant donor selection.
  • To assess the potential benefit of allele-level HLA matching in improving retransplant outcomes.

Main Methods:

  • Generation of T lymphocyte clones (TLC) using reciprocal priming with responder and stimulator cells expressing different DR11 microvariants.

Related Experiment Videos

  • Assessment of TLC recognition patterns to identify cellular crossreactivity among DR molecules.
  • Utilizing DNA-based HLA typing for allele-level identification.

    • Main Results:

    • A majority of TLCs recognized not only the specific DR11 allele but also DR molecules spanning other serologic specificities.
    • TLCs generated against specific DR11 microvariants showed crossreactivity with DR13, DR8, DR2, and DR4 alleles.
    • Significant crossreactivity patterns were observed, highlighting the limitations of serological matching for DR11.

    Conclusions:

    • Cellular crossreactivity among DR molecules, especially DR11 microvariants, is extensive and impacts retransplantation.
    • Excluding donors with strong in vitro cellular crossreactivity with previous donor molecules may improve retransplant outcomes.
    • Implementing allele-level HLA matching, particularly for diverse populations, holds promise for enhancing allograft survival in retransplant patients.