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Related Experiment Videos

Human eosinophils lack human leukocyte elastase

O Wiedow1, K Muhle, V Streit

  • 1Department of Dermatology, University of Kiel, Germany.

Biochimica Et Biophysica Acta
|April 12, 1996
PubMed
Summary
This summary is machine-generated.

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Human eosinophils do not contain significant levels of human leukocyte elastase. Previous findings suggesting their presence were likely due to neutrophil contamination in eosinophil samples.

Area of Science:

  • Immunology
  • Biochemistry
  • Cell Biology

Background:

  • Human leukocyte elastase (HLE) is a serine protease found in neutrophils.
  • Recent studies suggested HLE presence in human eosinophils, prompting further investigation.

Purpose of the Study:

  • To re-evaluate the presence and activity of human leukocyte elastase in human eosinophils.
  • To determine if eosinophils release active HLE.

Main Methods:

  • Quantification of active HLE in neutrophils and eosinophils.
  • Assay of spontaneous and stimulated elastase release from eosinophils.
  • Immunohistochemical analysis for HLE in eosinophils.

Main Results:

  • Neutrophils contained 2.5 pg of active HLE per cell.

Related Experiment Videos

  • Eosinophil elastase activity correlated directly with neutrophil contamination.
  • No spontaneous or stimulated HLE release was observed in eosinophils.
  • Immunohistochemistry showed no HLE immunoreactivity in eosinophils.

    • Conclusions:

    • Human eosinophils do not contain considerable amounts of human leukocyte elastase.
    • Previous reports of HLE in eosinophils are likely attributed to neutrophil contamination.