Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro evolution of thermodynamically stable turns

H X Zhou1, R H Hoess, W F DeGrado

  • 1DuPont Merck Pharmaceutical Company, Wilmington, Delaware 19880-0336, USA.

Nature Structural Biology
|May 1, 1996
PubMed
Summary

Protein primary structure influences turn formation. Stable protein structures favor specific turn sequences, indicating evolutionary pressure for thermodynamic stability in beta-turns.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Spontaneous remission of primary aldosteronism: a case series of three patients and literature review].

Zhonghua nei ke za zhi·2026
Same author

[Efficacy of venetoclax combined with hypomethylating agents in the treatment of patients newly diagnosed with core binding factor acute myeloid leukemia].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[Expert consensus on the treatment timing calender of congenital ear malformation with multiple malformations].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

[Emphasis the early diagnosis and treatment of plasma cell disease-related kidney disease].

Zhonghua yi xue za zhi·2025
Same author

[Predictors of adverse outcomes in patients with chronic obstructive pulmonary disease and pulmonary embolism and the predictive value of the simplified pulmonary embolism severity index].

Zhonghua yi xue za zhi·2024
Same author

[A multicenter study on effect of delayed chemotherapy on prognosis of Burkitt lymphoma in children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2024

Area of Science:

  • Protein structure and stability
  • Molecular biology
  • Biophysics

Background:

  • Protein primary structure dictates higher-order structures.
  • Beta-turns are crucial for protein folding and function.
  • The role of primary sequence in turn formation is not fully understood.

Purpose of the Study:

  • To investigate how primary structure dictates beta-turn formation.
  • To assess the impact of host protein stability on turn sequence selection.
  • To determine if evolutionary pressure favors thermodynamically stable turn structures.

Main Methods:

  • Substitution of native turn sequences with random sequences in host proteins.
  • Measurement of active protein fraction as a function of host stability and temperature.
  • Thermodynamic analysis of selected turn sequences.

Main Results:

  • Highly stable host proteins accommodated a higher fraction of functional random turn sequences.
  • Decreasing host stability and increasing temperature reduced the fraction of supporting sequences.
  • Selected turn sequences often resembled wild-type or known turn preferences.
  • Sequences selected under stringent conditions yielded the most stable proteins.

Conclusions:

  • Primary structure plays a significant role in specifying protein turns.
  • Beta-turns are under evolutionary selection for thermodynamic stability.
  • Protein stability is a key factor in the success of sequence variations in turns.

Related Experiment Videos