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4-1BB is expressed on CD45RAhiROhi transitional T cell in humans
B A Garni-Wagner1, Z H Lee, Y J Kim
1Department of Microbiology and Immunology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis, 46202-5120, USA.
Cellular Immunology
|April 10, 1996
Summary
This study developed a specific antibody (4B4-1) to detect human 4-1BB (CD137) on T cells. It reveals that human 4-1BB expression kinetics and T cell subset involvement differ from mouse models.
Area of Science:
- Immunology
- Cell Biology
Background:
- Murine 4-1BB (CD137) is a T cell co-stimulatory molecule crucial for T cell-mediated immune responses.
- Most research on 4-1BB has focused on mouse models, necessitating investigation into its human counterpart.
Purpose of the Study:
- To develop and validate a specific monoclonal antibody (mAb) for detecting human 4-1BB.
- To characterize the expression patterns of human 4-1BB on T cells following activation.
Main Methods:
- Production of mAbs against recombinant human 4-1BB (rh4-1BB).
- Flow cytometry (FCM) and immunoprecipitation assays to confirm antibody specificity and detect protein.
- Analysis of 4-1BB expression on peripheral blood T cells stimulated with phytohemagglutinin (PHA).
Main Results:
- The mAb 4B4-1 specifically binds rh4-1BB and detects 4-1BB on activated human T cells (CEM and peripheral blood T cells).
- Human 4-1BB expression is induced within 24 hours of PHA stimulation, peaking at 2-3 days, and occurs on both CD4+ and CD8+ T cell subsets.
- 4-1BB is expressed on both naive and memory T cell populations, with transitional cells emerging during activation.
Conclusions:
- The mAb 4B4-1 is a specific reagent for identifying human 4-1BB.
- Human 4-1BB exhibits distinct expression kinetics and T cell subset distribution compared to its murine counterpart, highlighting species-specific differences in T cell activation pathways.