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Generation of VV-hemorphin-7 from globin by peritoneal macrophages
N Dagouassat1, I Garreau, F Sannier
1Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences et Technologies, La Rochelle, France.
Abstract:
Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothesize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.