Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutagenic potential of binary and complex mixtures using different enzyme induction systems

K V Markiewicz1, L E Howie, S H Safe

  • 1Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, 77843-4458, USA.

Journal of Toxicology and Environmental Health
|April 5, 1996
PubMed
Summary

This study assessed the mutagenicity of chemical mixtures using rat liver enzyme systems (S9 fractions). Different enzyme inducers revealed the varying metabolic activation of mutagens, highlighting the role of cytochrome P-450 isozymes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aryl hydrocarbon receptor activity in intestinal epithelial cells in the formation of colonic tertiary lymphoid tissues.

American journal of physiology. Gastrointestinal and liver physiology·2024
Same author

Reduction of Mutagen Formation in Fried Ground Beef by Glandless Cottonseed Flour.

Journal of food protection·2019
Same author

Dietary and environmental estrogens and antiestrogens and their possible role in human disease.

Environmental science and pollution research international·2013
Same author

Effects of mineral nutrients, sludge application rate, and application frequency on biodegradation of two oily sludges.

Microbial ecology·2013
Same author

High-resolution PCB analysis: synthesis and chromatographic properties of all 209 PCB congeners.

Environmental science & technology·2012
Same author

Genotoxicity profiles and reaction characteristics of potassium polyethylene glycol dehalogenation of wood preserving waste.

Environmental science & technology·2011

Area of Science:

  • Environmental Toxicology
  • Chemical Mutagenesis
  • Drug Metabolism

Background:

  • Assessing the mutagenic potential of complex chemical mixtures is crucial for environmental and occupational health.
  • Liver enzyme systems, particularly cytochrome P-450 isozymes, play a critical role in metabolizing and detoxifying xenobiotics.
  • Inducible liver enzyme systems allow for the investigation of specific metabolic pathways involved in chemical activation or deactivation.

Purpose of the Study:

  • To investigate the mutagenic potential of binary and complex chemical mixtures.
  • To evaluate the influence of different inducible liver enzyme systems (S9 fractions) on mutagenicity.
  • To determine the role of specific cytochrome P-450 isozymes in the metabolic activation of mutagens.

Main Methods:

Related Experiment Videos

  • Preparation of Sprague-Dawley rat liver S9 fractions induced with Aroclor 1254 (AR), phenobarbital (PB), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), or uninduced (UI).
  • Mutagenicity testing using Salmonella typhimurium strain TA98 with various test chemicals and mixtures.
  • Test samples included benzo[a]pyrene (BaP), pentachlorophenol (PCP), binary mixtures, complex mixtures from industrial waste, and soil amendments.

    • Main Results:

    • Benzo[a]pyrene (BaP) showed significantly higher mutagenicity with AR- and TCDD-induced S9 fractions compared to PB- or UI-induced S9.
    • A complex mixture from wood-preserving waste-amended soil exhibited similar mutagenic responses across all S9 fractions.
    • A methanol extract from coal gasification waste demonstrated increased mutagenicity with AR- and TCDD-induced S9 compared to uninduced S9.

    Conclusions:

    • The study highlights the differential metabolic activation of mutagens and complex mixtures by various induced cytochrome P-450 isozymes.
    • Specific isozymes are critical for metabolizing certain chemicals, while others are important for complex mixture activation.
    • Understanding these metabolic pathways is essential for accurate risk assessment of environmental contaminants.