Related Experiment Video
Updated: Aug 9, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
A possible role for nitric oxide but not for prostaglandin E2 in basal and interleukin-1-beta-induced PRL release in
L Brunetti1, E Ragazzoni, P Preziosi
1Department of Pharmacology, G. D'Annunzio University, Chieti, Italy.
Abstract:
In previous experiments we have shown that nitric oxide (NO) was able to modulate CRH and ACTH release from cultured rat hypothalamic and anterior pituitary cells, in vitro. Now, we show experimental evidence of an involvement of NO in basal and interleukin-1 beta-induced prolactin (PRL) release. L-NG-nitro-arginine, an inhibitor of nitric oxide synthetase, and hemoglobin, a NO scavenger, impaired basal and interleukin-1-beta-induced PRL release, while molsidomine, a NO donor, was able to release PRL and to amplify interleukin-1-beta-induced PRL release, confirming a modulatory role for nitric oxide in pituitary hormone secretion. On the other hand, no evidence regarding a possible role of prostaglandin E2 (PGE2) in IL-1beta-induced PRL release came out from our experiments.
More Related Videos
Related Concept Videos
Paracrine Signaling
Amplifying Signals via Second Messengers
GPCRs Regulate Adenylyl Cylase Activity
Two...
IP3/DAG Signaling Pathway
Nitric Oxide Signaling Pathway
Acute Inflammation III: Local and Systemic Effects

